The InScriptase™ mRNA Synthesis 2X Master Mix: -CC/m1Ψ is an optimized, ready-to-use solution designed for efficient in vitro mRNA synthesis without co-transcriptional capping. This flexible 2X Master Mix contains all the essential components, including T7 RNA Polymerase and necessary cofactors, while allowing researchers to customize their mRNA synthesis by adding their preferred uridine (uridine, pseudouridine [Ψ], or N1-methylpseudouridine [m1Ψ]). Simply add your linearized DNA template and desired uridine variant for tailored mRNA production.

Key Features:

• Ease of Use: Simplified 2X Master Mix format—just add your linearized DNA template and uridine of choice.
• Flexible Uridine Incorporation: Allows users to customize the reaction with uridine, pseudouridine (Ψ), or N1-methylpseudouridine (m1Ψ) based on specific application needs.
• Uncapped mRNA Synthesis: Ideal for applications where co-transcriptional capping is not required, enabling downstream customization.
• High Yield and Consistency: Achieves up to 100 µg of mRNA per reaction from a 1 µg DNA input, ensuring reproducible and high-quality results.
• Versatile Applications: Suitable for a wide range of research needs, including biochemical assays, RNA structure-function studies, ribozyme synthesis, and custom RNA modifications.

Interested in trying out our 2x Mastermix? Use the “Generate Quote” button to receive a quote from one of our customer service representatives.