The InScriptase™ mRNA Synthesis 2X Master Mix: +CC/m1Ψ is an optimized, ready-to-use solution designed for rapid and efficient in vitro mRNA synthesis. This 2X Master Mix contains all the essential components, including T7 RNA Polymerase, nucleotides (featuring modified N1-methylpseudouridine [m1Ψ] for enhanced stability and reduced immunogenicity), capping reagents (CleanCap AG), and all necessary cofactors. Simply add your linearized DNA template to initiate high-yield mRNA production with minimal setup time.

Key Features:

• Ease of Use: Simplified 2X Master Mix format—just add your linearized DNA template.
• Enhanced mRNA Quality: Incorporates N1-methylpseudouridine (m1Ψ) for increased stability, reduced innate immune response, and improved translation efficiency.
• Efficient Capping: Includes co-transcriptional capping reagents for mRNA synthesis (CleanCap AG).
• High Yield and Consistency: Achieves up to 100 µg of mRNA per reaction from a 1 ug standard DNA input, ensuring reproducible, high-quality results.
• Versatile Applications: Delivers high-quality, transfection-grade mRNA suitable for cell-based assays and in vitro translation studies.

Designed to streamline your mRNA synthesis workflow, the InScriptase™ mRNA Synthesis 2X Master Mix: +CC/m1Ψ delivers reliable performance, high yields, and exceptional mRNA quality, saving time and simplifying your experimental setup.

Interested in trying out our 2x Mastermix? Use the “Generate Quote” button to receive a quote from one of our customer service representatives.